Mr Shashank V Gurjar MA (Hons) Cant MBA MRCS (Eng) MRCS (Ed) MSc Medical
Specialist Registrar in General Surgery (South East Thames circuit).
www.gmc-uk.org/about/register/panellists.aspKavisammelana
Asha Gurjar
www.arts.ed.ac.uk/sanskrit/13thWSC/3participants.htmlA day at Gokul Puri Bazaar
Gokul puri is an urban village situated on the northeast boundary of Delhi and counts Gurjar, as it’s the original inhabitants. But like other such villages that have lost their way into the entrails of urbanisation Gokulpuri is also a vast zigzag expanse of human cluster where migrants have out numbered the natives. My three visits to the village colony were no less than a revelation to me, because at the stage of writing the proposal, Gokulpuri had appeared to be a small point in the larger context of this demographic zone. But as I made progressive investigation into the field, the notion had to be substantially revised. It turned to be far bigger an area than i had expected. The fact that Gokul puri is a sort of frontline settlement to a vast area, which progressively merges with the territory of
UttarPradesh, enhances the importance of this market.
mail.sarai.net/pipermail/reader-list/2003-June/002837.htmlGurjar Abhijit Biogeochemistry and microbiology 2 BGM 12 28
www.essc.psu.edu/CECG_symposium/ECSS2007abstracts.pdfBGM - 12
Rapid Detection and Toxintyping of Clostridium perfringens Toxins in Feces
of Dairy Cattle by Real Time Multiplex PCR.
Gurjar, Abhijit1; Donaldson, Sarah2; Hegde, Narasimha3; Jayarao, Bhushan4.
1Dept. of Vet. and Biomedical Sci., Penn State University, aag172@psu.edu
2 Dept. of Vet. and Biomedical Sci., Penn State University, scd127@psu.edu
3 Dept. of Vet. and Biomedical Sci., Penn State University, nvh1@psu.edu
4 Dept. of Vet. and Biomedical Sci., Penn State University, bmj3@psu.edu
Clostridium perfringens is an anaerobic, gram positive, spore-forming bacteria with an arsenal of virulence factors
associated with a wide variety of diseases affecting most domestic animal species and humans. Conventional
bacteriology and PCR based confirmation techniques are currently employed to identify the toxinotypes of C.
perfringens. Our objectives were to reduce the time and increase the sensitivity and specificity of identifying C.
perfringens toxinotypes. We have developed dual-labeled fluorescence hybridization probe (TaqMan®) based real
time multiplex PCR assays for detection of C. perfringens toxin genes cpa, cpb, ia, etx, cpb2 and cpe directly from
cattle feces. The real time PCR assays were standardized using C. perfringens ATCC reference strains, field strains
of C. perfringens previously characterized, and other bacterial species. We designed the specific primers and probes
for the six toxin genes using published sequences from the NCBI database. Each real time assay was able to detect a
minimum genomic DNA quantity of 0.005 ìg for all the six toxin producing strains of C. perfringens. Real time
PCR assays with a serial 10-fold dilution of purified DNA yielded standard curves with high correlation coefficients.
No amplification was observed with DNA purified from bacterial species other than C. perfringens. A total of 307
fecal samples collected from 7 dairy herds in Pennsylvania were analyzed using the multiplex assays. The real time
PCR assays revealed that cpa, cpb, ia, etx, cpb2 and cpe were detected in 64 (21%), 6 (2%), 6 (2%), 4 (1.3%), 154
(50%) and 11 (3.6%) samples, respectively. A wide variation in the bacterial load of toxigenic C. perfringens in
feces from lactating cattle was observed by means of the range of cycle threshold values for PCR-positive samples
(ranging from 24 to 36 cycles). The findings of the study revealed that that C. perfringens beta2 toxin producing
strains are widely prevalent in lactating cows in Pennsylvania and they may play an important role in C. perfringens
associated diarrheal diseases.
